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Polar Body Genome Transfer for Preventing the Transmission of Inherited Mitochondrial Diseases (Jun 2014)

cell
Title:
Polar Body Genome Transfer for Preventing the Transmission of Inherited Mitochondrial Diseases
Journal:
Cell, Vol. 157, Issue 7, p1591–1604, 2014
Author(s):
Tian Wang, Hongying Sha, Dongmei Ji, Helen L. Zhang, Dawei Chen, Yunxia Cao, Jianhong Zhu
 

 

Short description:
Polar body genome transfer can effectively prevent the transmission of defective mitochondria DNA across generations, indicating the potential of this procedure to treat inherited mitochondrial diseases.
Link to the journal
 

 

Abstract taken from PubMed

Abstract:
Inherited mtDNA diseases transmit maternally and cause severe phenotypes. Currently, there is no effective therapy or genetic screens for these diseases; however, nuclear genome transfer between patients' and healthy eggs to replace mutant mtDNAs holds promises. Considering that a polar body contains few mitochondria and shares the same genomic material as an oocyte, we perform polar body transfer to prevent the transmission of mtDNA variants. We compare the effects of different types of germline genome transfer, including spindle-chromosome transfer, pronuclear transfer, and first and second polar body transfer, in mice. Reconstructed embryos support normal fertilization and produce live offspring. Importantly, genetic analysis confirms that the F1 generation from polar body transfer possesses minimal donor mtDNA carryover compared to the F1 generation from other procedures. Moreover, the mtDNA genotype remains stable in F2 progeny after polar body transfer. Our preclinical model demonstrates polar body transfer has great potential to prevent inherited mtDNA diseases.
Link to the paper on PubMed
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