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Localized, Reactive F-Actin Dynamics Prevents Abnormal Somatic Cell Penetration by Mature Spermatids (Sep 2016)

cell
Title:
Localized, Reactive F-Actin Dynamics Prevents Abnormal Somatic Cell Penetration by Mature Spermatids
Journal:
Dev Cell. 2016 Sep 12;38(5):507-21. doi: 10.1016/j.devcel.2016.07.001. Epub 2016 Aug 18.
Author(s):
Dubey P1, Shirolikar S1, Ray K2
Author(s) affiliation:
1Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India.
2Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India. Electronic address: This email address is being protected from spambots. You need JavaScript enabled to view it..
 

 

Short description: Highlights
*Mature spermatids are released from the cyst enclosure tail-first
*F-actin dynamics prevents abnormal penetration of the cyst cell by spermatids
*Aberrant spermatid head intrusion induces F-actin assembly in the somatic cell
*WASp-Arp2/3 recruitment induces the F-actin assembly averting the cell penetration
Link to the journal
 

 

Abstract taken from PubMed

Abstract:
Spermatogenesis occurs inside a somatic cell enclosure. Sperm release, the most important final step and a target for contraceptives, has been extensively studied in fixed tissue preparations. Here, we provide a time-lapse description of the release process in Drosophila testis ex vivo. We show that the spermatid tails exit the somatic enclosure and enter the testicular duct first, followed by the spermatid heads. Prior to this, individual spermatid heads attempt to invade the head cyst cell, and on each occasion they are repelled by a rapid and local F-actin polymerization response from the head cyst cell. The F-actin assembly involves N-WASp, D-WIP, and Arp2/3 complex and dissipates once the spermatid head retreats back into the fold. These findings revise the existing spermiation model in Drosophila and suggest that somatic cells can actively oppose mechanical cell invasion attempts using calibrated F-actin dynamics in situ.

Copyright © 2016 Elsevier Inc. All rights reserved.
Link to the paper on PubMed
 




 

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