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Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency (Apr 2017)

cell
Title:
Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency
Journal:
Cell Volume 169, Issue 2, p243–257.e25, 6 April 2017
Author(s):
Yang Y1, Liu B1, Xu J2, Wang J2, Wu J3, Shi C4, Xu Y5, Dong J2, Wang C2, Lai W5, Zhu J2, Xiong L5, Zhu D1, Li X2, Yang W6, Yamauchi T3, Sugawara A3, Li Z3, Sun F7, Li X7, Li C8, He A8, Du Y2, Wang T2, Zhao C2, Li H2, Chi X9, Zhang H9, Liu Y10, Li C11, Duo S12, Yin M6, Shen H13, Belmonte JC14, Deng H15
Author(s) affiliation:
1 Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China; Shenzhen Stem Cell Engineering Laboratory, Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen 518055, China.
2 Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
3 Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Rd., La Jolla, CA 92037, USA.
4Reproductive Medical Center, Peking University People's Hospital, Peking University, Beijing, 100044, China.
5Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, College of Life Sciences, Peking University, Beijing 100871, China.
6 Beijing Vitalstar Biotechnology, Beijing 100012, China.
7College of Animal Science and Technology, Hebei University, Baoding 071002, China.
8 Institute of Molecular Medicine, Peking University, PKU-Tsinghua U Joint Center for Life Sciences, Beijing 100871, China.
9 Laboratory of Stem Cells, Development and Reproductive Medicine, Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
10School of Life Sciences, Tsinghua University, Beijing 100084, China.
11Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; School of Life Sciences, Center for Statistical Science, Peking University, Beijing 100871, China; Center for Bioinformatics, Peking University, Beijing 100871, China.
12 Institute of Zoology, Chinese Academy Sciences, Beijing 100101, China.
13Reproductive Medical Center, Peking University People's Hospital, Peking University, Beijing, 100044, China. Electronic address: This email address is being protected from spambots. You need JavaScript enabled to view it..
14Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Rd., La Jolla, CA 92037, USA. Electronic address: This email address is being protected from spambots. You need JavaScript enabled to view it..
15Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China; Shenzhen Stem Cell Engineering Laboratory, Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen 518055, China. Electronic address: This email address is being protected from spambots. You need JavaScript enabled to view it..
 

 

Highlights:
•A chemical cocktail enables derivation of EPS cells from both humans and mice
•A single mouse EPS cell generates both embryonic and extraembryonic lineages in vivo
•Mouse EPS cells show robust and superior chimeric ability at the single cell level
•Human EPS cells show interspecies chimeric competency in mouse conceptuses
Link to the journal
 

 

Abstract taken from PubMed

Abstract:
Of all known cultured stem cell types, pluripotent stem cells (PSCs) sit atop the landscape of developmental potency and are characterized by their ability to generate all cell types of an adult organism. However, PSCs show limited contribution to the extraembryonic placental tissues in vivo. Here, we show that a chemical cocktail enables the derivation of stem cells with unique functional and molecular features from mice and humans, designated as extended pluripotent stem (EPS) cells, which are capable of chimerizing both embryonic and extraembryonic tissues. Notably, a single mouse EPS cell shows widespread chimeric contribution to both embryonic and extraembryonic lineages in vivo and permits generating single-EPS-cell-derived mice by tetraploid complementation. Furthermore, human EPS cells exhibit interspecies chimeric competency in mouse conceptuses. Our findings constitute a first step toward capturing pluripotent stem cells with extraembryonic developmental potentials in culture and open new avenues for basic and translational research. VIDEO ABSTRACT.

Copyright © 2017 Elsevier Inc. All rights reserved.
Link to the paper on PubMed
 




 

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