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Embryo catheter loading and embryo culture techniques: results of a worldwide web-based survey (Aug 2014)

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Title:
Embryo catheter loading and embryo culture techniques: results of a worldwide web-based survey
Journal:
Journal of Assisted Reproduction and Genetics, August 2014, Volume 31, Issue 8, pp 1029-1036
Author(s):
Christianson MS1, Zhao Y2, Shoham G3, Granot I4, Safran A5, Khafagy A6, Leong M, Shoham Z7.
Author(s) affiliation:
1Department of Gynecology and Obstetrics, Division of Reproductive Endocrinology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
2Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
3Department of Obstetrics and Gynecology, Kaplan Medical Center, Rehovot, 76100, Israel
4European Center for Motherhood, Warsaw, Poland
5Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA
6IVF Centre, The Hong Kong Sanatorium and Hospital, Happy Valley, Hong Kong
7Department of Obstetrics and Gynecology, Kaplan Medical Center, Rehovot, 76100, Israel
 

 

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Abstract taken from PubMed

Purpose:
To identify trends in embryo catheter loading and embryo culture techniques performed worldwide.
Methods:
A retrospective evaluation using the results of a web-based survey, (IVFWorldwide ( www.IVF-worldwide.com ), was performed
Results:
Responses from 265 centers in 71 countries were obtained. Most centers (97 %) prefered a catheter with its orifice on top, with only 3 % preferring a catheter with the orifice on its side; 41 % preferred a catheter marked for clear ultrasound view. The most commonly-reported methods of embryo loading were medium-air-embryo-airmedium (42 %), medium in catheter with embryo at end (20 %) and medium-air-embryo (15 %). In 68 % of centers the final volume of the catheter was up to 0.3 ml, with only 19 % using 0.3-0.5 ml and 1 % using 0.5-0.7 ml. Using reduced oxygen concentrations for embryo culture was divided between those who used it in combination with the twogas system (34 %) and those who did not use it at all (39 %); 24 % reported using a three-gas system. Most clinics using reduced oxygen concentrations used it throughout the entire culture period. Half of centers (51 %) reported using reduced oxygen concentrations for the entire IVF population while 6 % reserved it only for blastocyst transfer. The use of sequential media was highly dominant with 40 % reporting its use.
Link to the paper on PubMed
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