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GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells (Mar 2014)

plos one
Title:
GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells
Journal:
PLoS One. 2014 Mar 6;9(3)
Author(s):
Jigal Haas, Libby Ophir, Eran Barzilay, Gil M. Yerushalmi, Yuval Yung, Alon Kedem, Ettie Maman, Ariel Hourvitz
 

 

Short description:
Link to the journal
 

 

Abstract taken from PubMed

Objective:
To investigate the mRNA expression of genes related to steroidogenesis and OHSS in granulosa cells (GCs) of patients triggered with GnRH agonist compared to patients triggered with hCG.
Design:
Mural GCs were obtained at the time of oocyte retrieval and gene expression was analyzed using quantitative real time RT-PCR.
Settings:
Single center, case control study.
Patient(s):
24 women who were treated with GnRH agonist or hCG for triggering of ovulation.
Interventions:
GC collection.
Main outcome measure(s):
The expression of genes related to steroidogenesis and OHSS in mural GCs.
Results:
The fertilization rate was similar in the two groups. The mRNA expression of CYP19A1 (0.50 vs 1, arbitrary unit), CYP11A1 (0.6 vs. 1) and 3 beta hydroxysteroid-dehydrogenase (0.39 vs 1) was significantly lower in the GnRH group. The expression of VEGF (0.74 vs. 1) and inhibin β B (0.38 vs 1) was lower in the GnRH analog triggered group.
Conclusions:
Expression of genes related to steroidogenesis is lower at the time of oocyte retrieval in patients triggered with GnRH agonist. The decreased expression of VEGF and inhibin β B in the GnRH agonist group can explain the mechanism of early OHSS prevention.
Link to the paper on PubMed
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