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Preimplantation genetic screening (PGS) : what is my opinion

The purpose of the current survey is to evaluate the extent and patterns of use of PGS worldwide, and to gain an insight on the views and opinions of the ART community on the use of PGS.
This survey was suggested and composed by Ariel Wiessman, Yuval Yaron and Simon Fishel.

Is preimplantation genetic diagnosis for aneuploidy screening (PGS) currently being used in your clinic?

 

YES

Which patients are being offered to include PGS in their treatment cycle? (multiple answers allowed)
  • Patients with advanced maternal age (>35 years)
  • Patients with repeated implantation failure
  • Patients with recurrent abortions and normal karyotype
  • Good prognosis patients
  • All patients in your clinic

 

Is ovarian reserve a major factor in the inclusion criteria for PGS in your clinic?
  • Yes
  • No

 

Patients with a low ovarian reserve are excluded
  • Yes
  • No

 

All patients are included irrespective of ovarian reserve
  • Yes
  • No

 

A minimum of embryos/blastocysts are necessary for inclusion irrespective of ovarian reserve
  • Yes
  • No

 

To what extent is PGS being used in your clinic?
  • Very few
  • Less than 10% of cycles
  • 10-20% of cycles
  • 20-30% of cycles
  • 30-40% of cycles
  • >50% of cycles

 

Who is responsible for the funding of the PGS part of the IVF cycle?
  • Patients are paying out of pocket
  • PGS is covered by the patients' insurance
  • PGS is funded by public sector
  • Other

 

At what stage of development are the majority embryo biopsies being performed in your clinic?
  • Polar body biopsy (PB1 and PB2)
  • Polar body biopsy (PB1 only)
  • Blastomere biopsy
  • Trophectoderm biopsy (Day 5 only)
  • Trophectoderm biopsy (Day 5 and 6 depending on stage of blastocyst)
  • None of the above

 

Do you perform PGS on frozen thawed embryos if patient failed a non PGS cycle?
  • Yes
  • No

 

Which method of genetic testing is predominantly used in your clinic for determination of embryo ploidy status?
  • Array CGH
  • Real-time quantitative PCR
  • SNP microarray
  • Next generation sequencing
  • FISH
  • Other
  • I don't know

 

Where is genetic testing being performed?
  • In-house genetic laboratory
  • Centralized/referral laboratory
  • Both

 

Which method of embryo transfer is preferred in your clinic following PGS?
  • Fresh embryo transfer
  • Frozen-thawed embryo transfer
  • Both

 

How many euploid embryos do you normally transfer?
  • Single embryo transfer to all patients
  • Double embryo transfer to all patients
  • Single embryo transfer only to good prognosis patients
  • Decision is made based on patients' request

 

To what extent do you think that PGS is evidence-based?
  • Clearly evidence based
  • Most likely evidence based
  • Undecided
  • Probably not evidence based
  • Not evidence based

 

Do you think that more randomized trials are needed to support the use of PGS?
  • Yes
  • No

 

Do you believe in PGS to: (multiple answers allowed)
  • Improve live birth rate only
  • Reduce miscarriage rate only
  • To prevent the transfer of aneuploid embryos only
  • None of the above

 

Is PGS regulated in your country?
  • Yes - Number of embryos transferred after PGS is also regulated
  • No - Clinical freedom on the number of embryos to transfer
  • None of the above

 

NO

Is PGS done by other IVF units in your country?
  • Yes
  • No

 

Why is PGS not being performed in your clinic? (multiple answers allowed)
  • Lack of techniques/skilled staff and instrumentation
  • Lack of demand by patients (including financial considerations)
  • Investment cost
  • It is illegal in my country
  • Other

 

 

Do you think it is clinically justified to offer PGS at the moment
  • Yes
  • No

 

If PGS were available, which patients should be offered to include PGS in their treatment cycle? (multiple answers allowed)
  • Patients with advanced maternal age (>35 years)
  • Patients with repeated implantation failure
  • Patients with recurrent abortions and normal karyotype
  • Good prognosis patients
  • All patients
  • None of the above

 

Should ovarian reserve be a major factor in the inclusion criteria for PGS? (multiple answers allowed)
  • Patients with a low ovarian reserve should be excluded
  • All patients should be included irrespective of ovarian reserve
  • A minimum of embryos/blastocysts should be necessary for inclusion irrespective of ovarian reserve
  • None of the above