- Title:
- A microtubule-organizing center directing intracellular transport in the early mouse embryo
- Journal:
- Science 01 Sep 2017: Vol. 357, Issue 6354, pp. 925-928
- Author(s):
- Zenker J1, White MD1, Templin RM2, Parton RG2, Thorn-Seshold O 3, Bissiere S1, Plachta N 4,5
- Author(s) affiliation:
- 1Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore.
2 Institute for Molecular Biosciences and Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland, Australia.
3Department of Pharmacy, Center for Drug Research, Ludwig-Maximilians-University Munich, Munich, Germany.
4Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore
5Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
- Short description:
- The centrosome is the primary microtubule-organizing center (MTOC) of most animal cells; however, this organelle is absent during early mammalian development. Therefore, the mechanism by which the mammalian embryo organizes its microtubules (MTs) is unclear. We visualize MT bridges connecting pairs of cells and show that the cytokinetic bridge does not undergo stereotypical abscission after cell division. Instead, it serves as scaffold for the accumulation of the MT minus-end–stabilizing protein CAMSAP3 throughout interphase, thereby transforming this structure into a noncentrosomal MTOC. Transport of the cell adhesion molecule E-cadherin to the membrane is coordinated by this MTOC and is required to form the pluripotent inner mass. Our study reveals a noncentrosomal form of MT organization that directs intracellular transport and is essential for mammalian development.
- Link to the journal
Abstract taken from PubMed
- Abstract:
- The centrosome is the primary microtubule-organizing center (MTOC) of most animal cells; however, this organelle is absent during early mammalian development. Therefore, the mechanism by which the mammalian embryo organizes its microtubules (MTs) is unclear. We visualize MT bridges connecting pairs of cells and show that the cytokinetic bridge does not undergo stereotypical abscission after cell division. Instead, it serves as scaffold for the accumulation of the MT minus-end-stabilizing protein CAMSAP3 throughout interphase, thereby transforming this structure into a noncentrosomal MTOC. Transport of the cell adhesion molecule E-cadherin to the membrane is coordinated by this MTOC and is required to form the pluripotent inner mass. Our study reveals a noncentrosomal form of MT organization that directs intracellular transport and is essential for mammalian development.
Copyright © 2017, American Association for the Advancement of Science. - Link to the paper on PubMed
