Before vitrification, blastocoelic fluid (0.3-0.5 nl) is withdrawn from human blastocysts by microsuction and (usually) discarded. The metabolomic of blastocoele fluid was studied using Rapid Resolution-Reverse Phase (RR-RP) and mass spectrometry coupled to HPLC (RR-RP-HPLC-ESI-MS). The characterization of DNA fragments present in this fluid, and the evaluation of these fragments as potential target for Preimplantation Genetic Diagnosis was performed by using, real-time PCR based on SYBR green chemistry, Whole Genome Amplification techniques and -subsequently- Next Generation Sequencing approaches.

Aneuploidism , monogenic diseases and possibly embryo markers of its own viability could be detected in the blastocoele to improve embryo transfer efficiency.

Data will be presented at the
OVARIAN CLUB III
The Inverse Pyramid: Regulating Follicle Number and Oocyte Quality
Paris, France • November 14 - 17, 2013
http://www.comtecmed.com/oc/2013/pre_program.aspx